Following incubation, if the organism is phenylalanine deaminase positive, a green color develops after adding which reagent?

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Prepare for the Clinical Laboratory Science Bacteriology Test. Engage with multiple choice questions and flashcards with helpful hints and explanations. Ace your exam confidently!

Multiple Choice

Following incubation, if the organism is phenylalanine deaminase positive, a green color develops after adding which reagent?

Explanation:
Phenylalanine deaminase acts on phenylalanine to produce phenylpyruvic acid. After incubation, adding ferric chloride reacts with the phenylpyruvic acid to form a green (olive-green) color, indicating a positive PAD result. The green color comes from the ferric chloride complex with phenylpyruvic acid, signaling enzyme activity. The other reagents listed are not used in this test and would not produce this green color, as they pertain to different biochemical assays.

Phenylalanine deaminase acts on phenylalanine to produce phenylpyruvic acid. After incubation, adding ferric chloride reacts with the phenylpyruvic acid to form a green (olive-green) color, indicating a positive PAD result. The green color comes from the ferric chloride complex with phenylpyruvic acid, signaling enzyme activity. The other reagents listed are not used in this test and would not produce this green color, as they pertain to different biochemical assays.

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