Using the rapid chromogenic cephalosporin method, a positive beta-lactamase result is indicated by which color change?

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Prepare for the Clinical Laboratory Science Bacteriology Test. Engage with multiple choice questions and flashcards with helpful hints and explanations. Ace your exam confidently!

Multiple Choice

Using the rapid chromogenic cephalosporin method, a positive beta-lactamase result is indicated by which color change?

Explanation:
The rapid chromogenic cephalosporin test uses nitrocefin, a cephalosporin substrate that changes color when its beta-lactam ring is broken by beta-lactamase. Unhydrolyzed nitrocefin is yellow, and when beta-lactamase hydrolyzes it, the product turns red. Therefore, a red color indicates beta-lactamase activity (a positive result), while staying yellow would indicate no beta-lactamase activity. Green or blue aren’t part of this substrate’s positive readout.

The rapid chromogenic cephalosporin test uses nitrocefin, a cephalosporin substrate that changes color when its beta-lactam ring is broken by beta-lactamase. Unhydrolyzed nitrocefin is yellow, and when beta-lactamase hydrolyzes it, the product turns red. Therefore, a red color indicates beta-lactamase activity (a positive result), while staying yellow would indicate no beta-lactamase activity. Green or blue aren’t part of this substrate’s positive readout.

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