Which non-lactose fermenting organism does not produce fluorescein?

Prepare for the Clinical Laboratory Science Bacteriology Test. Engage with multiple choice questions and flashcards with helpful hints and explanations. Ace your exam confidently!

Multiple Choice

Which non-lactose fermenting organism does not produce fluorescein?

Explanation:
Fluorescein (pyoverdin) production is a pigment trait that helps differentiate non-lactose–fermenting Gram-negative rods in the Pseudomonas group. Many Pseudomonas species synthesize fluorescein and will glow under UV light due to this pigment, so organisms like Pseudomonas aeruginosa, Pseudomonas fluorescens, and Pseudomonas putida typically show fluorescence. Alcaligenes, on the other hand, does not produce this pigment, so its colonies do not fluoresce. Therefore, the non-lactose–fermenting organism that does not produce fluorescein is Alcaligenes.

Fluorescein (pyoverdin) production is a pigment trait that helps differentiate non-lactose–fermenting Gram-negative rods in the Pseudomonas group. Many Pseudomonas species synthesize fluorescein and will glow under UV light due to this pigment, so organisms like Pseudomonas aeruginosa, Pseudomonas fluorescens, and Pseudomonas putida typically show fluorescence. Alcaligenes, on the other hand, does not produce this pigment, so its colonies do not fluoresce. Therefore, the non-lactose–fermenting organism that does not produce fluorescein is Alcaligenes.

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