Which of the following is catalase negative?

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Prepare for the Clinical Laboratory Science Bacteriology Test. Engage with multiple choice questions and flashcards with helpful hints and explanations. Ace your exam confidently!

Multiple Choice

Which of the following is catalase negative?

Explanation:
The main idea being tested is whether an organism has the catalase enzyme, detected by the catalase test. When you apply hydrogen peroxide to a colony, bubbles indicate catalase activity because the enzyme rapidly breaks down hydrogen peroxide into water and oxygen; no bubbles mean the organism is catalase negative. In this set, Bacillus, Corynebacterium, and Listeria all produce catalase, so they will show bubbling. Leuconostoc, on the other hand, does not produce catalase, so there is no bubbling. This makes Leuconostoc catalase negative, which helps distinguish it from the other organisms in identification workflows.

The main idea being tested is whether an organism has the catalase enzyme, detected by the catalase test. When you apply hydrogen peroxide to a colony, bubbles indicate catalase activity because the enzyme rapidly breaks down hydrogen peroxide into water and oxygen; no bubbles mean the organism is catalase negative. In this set, Bacillus, Corynebacterium, and Listeria all produce catalase, so they will show bubbling. Leuconostoc, on the other hand, does not produce catalase, so there is no bubbling. This makes Leuconostoc catalase negative, which helps distinguish it from the other organisms in identification workflows.

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